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Literature summary extracted from

  • Lee, T.Y.; Park, Y.S.; Garcia, G.A.; Sunahara, R.K.; Woods, J.H.; Yang, V.C.
    Cell permeable cocaine esterases constructed by chemical conjugation and genetic recombination (2012), Mol. Pharm., 9, 1361-1373.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.84 expressed in Escherichia coli BL21 Star (DE3) cells Rhodococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.84 LMWP-S-S-T172R/G173Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Rhodococcus sp.
3.1.1.84 LMWP-T172R/G173Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Rhodococcus sp.
3.1.1.84 T172R/G173Q -LMWP the mutant shows reduced catalytic efficiency compared to the wild type enzyme Rhodococcus sp.
3.1.1.84 T172R/G173Q -YGRKKRRQRRR the mutant shows reduced catalytic efficiency compared to the wild type enzyme Rhodococcus sp.
3.1.1.84 YGRKKRRQRRR-T172R/G173Q the mutant still maintains 52% of its cocaine-hydrolyzing efficiency even after incubation at 37°C for 24 h Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.84 0.0288
-
 cocaine wild type enzyme, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0305
-
 cocaine mutant enzyme LMWP-S-S-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0306
-
 cocaine mutant enzyme T172R/G173Q -YGRKKRRQRRR, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0396
-
 cocaine mutant enzyme YGRKKRRQRRR-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0415
-
 cocaine mutant enzyme T172R/G173Q -LMWP, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.049
-
 cocaine mutant enzyme LMWP-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.84 70000
-
 x * 70000, SDS-PAGE Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.84 cocaine + H2O Rhodococcus sp.
-
 ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O Rhodococcus sp. MB1
-
 ecgonine methyl ester + benzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.84 Rhodococcus sp. Q9L9D7
-
-
3.1.1.84 Rhodococcus sp. MB1 Q9L9D7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.84 Talon metal affinity column chromatography and Q-Sepharose column chromatography Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.84 cocaine + H2O
-
 Rhodococcus sp. ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O
-
 Rhodococcus sp. MB1 ecgonine methyl ester + benzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.84 ? x * 70000, SDS-PAGE Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.84 cocE
-
 Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.84 37
-
 the in vitro half-life at 37°C is about 13.7 min Rhodococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.84 31.45
-
 cocaine mutant enzyme T172R/G173Q -YGRKKRRQRRR, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 32.62
-
 cocaine mutant enzyme YGRKKRRQRRR-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 34.42
-
 cocaine mutant enzyme LMWP-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 35.17
-
 cocaine mutant enzyme T172R/G173Q -LMWP, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 39.17
-
 cocaine mutant enzyme LMWP-S-S-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 44.85
-
 cocaine wild type enzyme, at pH 7.4 and 37°C Rhodococcus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.84 702
-
 cocaine mutant enzyme LMWP-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 823
-
 cocaine mutant enzyme YGRKKRRQRRR-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 847
-
 cocaine mutant enzyme T172R/G173Q -LMWP, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 1028
-
 cocaine mutant enzyme T172R/G173Q -YGRKKRRQRRR, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 1283
-
 cocaine mutant enzyme LMWP-S-S-T172R/G173Q, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 1560
-
 cocaine wild type enzyme, at pH 7.4 and 37°C Rhodococcus sp.